SMALP network

SMALP-related publications by year

smalp pubs

Each paper (+ password protected PDF) is related to SMALPs and native nanodiscs:

2023 · 2022 · 2021 · 2020 · 2019 · 2018 · 2017· 2016 · 2015


Kervin TA, Overduin M. Membranes are functionalized by a proteolipid code. BMC Biology, 22:46. PDF

Wang X, Slater A, Lee SC, Harrison N, Pollock NL, Bakker SE, Navarro S, Nieswandt B, Dafforn TR, García Á, Watson SP, Tomlinson MG. Purification and characterisation of the platelet-activating GPVI/FcRγ complex in SMALPs. Arch Biochem Biophys.109944, in press. PDF

Ayub H, Murray RJ, Kuyler GC, Napier-Khwaja F, Gunner J, Dafforn TR, Klumperman B, Poyner DR, Wheatley M. GPCRs in the round: SMA-like copolymers and SMALPs as a platform for investigating GPCRs. Arch Biochem Biophys. 109946, in press. PDF

Overduin M, Bhat R. Recognition and remodeling of endosomal zones by sorting nexins. BBA Biomembr, 1866: 184305, in press. PDF

Das S, Negi S. A novel strategy for partial purification of alkane hydroxylase from P. chrysogenum SNP5 through reconstituting its native membrane into liposome. Sci Rep. 14(1):3779. PDF

Tutkus M, Lundgaard CV, Veshaguri S, Tønnesen A, Hatzakis N, Rasmussen SGF, Stamou D. Probing Activation and Conformational Dynamics of the Vesicle-Reconstituted β2 Adrenergic Receptor at the Single-Molecule Level. J Phys Chem B. in press. PDF

Kislova S, Motov V, Myasnyanko I, Pytskii I, Goncharuk S, Boldyrev I. Conformational transitions of maleic acid segment drive pH induced changes in SMA polymer structure and solubility J Mol Liq 398, 124302. PDF

Brown C, Ghosh S, McAllister R, Kumar M, Walker GSun E, Aman T, Panda A, Kumar S, Li W, Coleman J, Liu Y, Rothman JE, Bhatacharyya M, Gupta K. A proteome-wide quantitative guide for nanoscale spatially resolved extraction of membrane proteins into native nanodiscs. bioRxiv. PDF

van Hilten N, Verwei N, Methorst J, Nase C, Bernatavicius A, Risselada, HJ, Membrane facial recognition: How proteins actually see cholesterol. Bioinformatics. in press. PDF

Tabefam M, Smith MD, Jelokhani-Niaraki M. Expression, purification and folding of native like mitochondrial carrier proteins in lipid membranes. Front Biophys. in press. PDF

Ullrich J, Haueis L, Ohlhoff C, Zemella A, Kubick S, Stech M. Solubilization of Oligomeric Cell-Free Synthesized Proteins Using SMA Copolymers. Methods Mol Biol. 2762:293-308. PDF

Woubshete M, Cioccolo S, Byrne B. Advances in membrane mimetic systems for manipulation and analysis of membrane proteins; detergents, polymers, lipids and scaffolds. Chempluschem. e202300678. PDF

Brisson ERL, Worthington MJH, Kerai S, Müllner M. Nanoscale polymer discs, toroids and platelets: a survey of their syntheses and potential applications. Chem Soc Rev. in press. PDF

Ekundayo B, Arullampalam P, Gerber CE, Hämmerli AF, Guichard S, Boukenna M, Ross D, Lochner M, Rougier JS, Stahlberg H, Abriel H, Ni D, Identification of a Binding Site for Small Molecule Inhibitors Targeting Human TRPM4. bioRxiv. PDF

Chu C, Vargas C, Barbosa MC, Sommerhage S, Schröder GF, Keller S, Etzkorn M. Capturing G protein-coupled receptors into native lipid-bilayer nanodiscs using new diisobutylene/maleic acid (DIBMA) copolymers, bioRxiv. PDF

Selvasingh JA, McDonald EF, Mckinney JR, Meiler J, Ledwitch KV. Dark nanodiscs as a model membrane for evaluating membrane protein thermostability by differential scanning fluorimetry. Biophys J. in press. PDF

Yoon S, Bae HE, Hariharan P, Nygaard A, Lan B, Woubshete M, Sadaf A, Liu X, Loland CJ, Byrne B, Guan L, Chae PS. Rational Approach to Improve Detergent Efficacy for Membrane Protein Stabilization. Bioconjug Chem. in press. PDF

Pata J, Moreno A, Wiseman B, Magnard S, Lehlali I, Dujardin M, Banerjee A, Högbom M, Boumendjel A, Chaptal V, Prasad R, Falson P. Purification and characterization of Cdr1, the drug-efflux pump conferring azole resistance in Candida species. Biochimie. 220:167-178. PDF

Moller E, Britt M, Zhou F, Yang H, Ernst R, Sukharev S, Matthies D. Polymer-solubilized structure of the mechanosensitive channel MscS suggests the role of protein-lipid interactions in the functional gating cycle bioRxiv. PDF

Williamson G, Bizior A, Harris T, Pritchard L, Hoskisson PA, Javelle A. Biological ammonium transporters from the Amt/Mep/Rh superfamily: mechanism, energetics, and technical limitations. Biosci Rep. 44(1) BSR20211209. PDF

Williamson G, Harris T, Bizior A, Hoskisson PA, Pritchard L, Javelle A. Biological ammonium transporters: evolution and diversification. FEBS J. in press. PDF

Barrantes FJ. Modulation of a rapid neurotransmitter receptor-ion channel by membrane lipids. Front Cell Dev Biol. 11:1328875. PDF

Al Huq MS, Raja K, Oviya IR. Prokaryotic cell membrane‑based protein technologies. World Acad Sci J. 6(2): 12. PDF

Dalal V, Arcario MJ, Petroff JT 2nd, Tan BK, Dietzen NM, Rau MJ, Fitzpatrick JAJ, Brannigan G, Cheng WWL. Lipid nanodisc scaffold and size alter the structure of a pentameric ligand-gated ion channel. Nat Commun. 15(1):25. PDF

Galiakhmetov AR, Shah AA, Lane A, Davern CM, Proulx C, Nevzorov AA. Peptoid-based macrodiscs of variable lipid composition for structural studies of membrane proteins by oriented-sample solid-state NMR. J Struct Biol X. 9:100095. PDF

Krajewska M, Możajew M, Filipek S, Koprowski P. Interaction of ROMK2 channel with lipid kinases DGKE and AGK: Potential channel activation by localized anionic lipid synthesis. BBA Mol Cell Biol Lipids.1869(2):159443. PDF

Walker G, Brown C, Ge X, Kumar S, Muzumdar MD, Gupta K, Bhattacharyya M. Oligomeric organization of membrane proteins from native membranes at nanoscale spatial and single-molecule resolution. Nat Nanotechnol. in press. PDF

Mitra K, Krishnan Y. Lightly counting membrane proteins in native nanodiscs. Nat Nanotechnol. in press. PDF

Mu Q, Deng H, An X, Liu G, Liu C. Designing nanodiscs as versatile platforms for on-demand therapy. Nanoscale. in press PDF

Krishnarjuna B, Sharma G, Ravula T, Ramamoorthy A. Factors influencing the detergent-free membrane protein isolation using synthetic nanodisc-forming polymers. BBA Biomembr. 1866(1):184240. PDF

Deutz LN, Sarıkaya S, Dickinson DJ. Membrane extraction in native lipid nanodiscs reveals dynamic regulation of Cdc42 complexes during cell polarization. Biophys press. PDF

Rezaeeyan H, Yari F and Milani S. The Blood Group Rhc Protein from Human Erythrocyte Membranes as an Immunogen for Producing Antibodies in MiceIJPHO 14.1: 44-52. PDF


Adair BD, Xiong JP, Yeager M, Arnaout MA. Cryo-EM structures of full-length integrin αIIbβ3 in native lipids. Nat Commun. 14(1):4168. PDF

Thomas B, Chockalingam K, Chen Z. Methods for Engineering Binders to Multi-Pass Membrane Proteins. Bioengineering. 10(12):1351. PDF

Neville GM, Morrison KA, Shilliday ER, Doutch J, Dalgliesh R, Price GJ, Edler KJ. The effect of polymer end-group on the formation of styrene - maleic acid lipid particles (SMALPs). Soft Matter. 19(44):8507-8518. PDF

Hoang Trinh TK, Catalano C, Guo Y. Fabrication of membrane proteins in the form of native cell membrane nanoparticles using novel membrane active polymers. Nanoscale Adv. 5(21):5932-5940. PDF

Workman CE, Bag P, Cawthon B, Ali FH, Brady NG, Bruce BD, Long BK. Alternatives to Styrene- and Diisobutylene-Based Copolymers for Membrane Protein Solubilization via Nanodisc Formation. Angew Chem Int Ed Engl. 62(43):e202306572. PDF

Venkataraghavan A, Schwerdt JG, Tyerman SD, Hrmova M. Barley Nodulin 26-like intrinsic protein permeates water, metalloids, saccharides, and ion pairs due to structural plasticity and diversification. J Biol Chem. 299(12):105410. PDF

Peclo, M., Lipatova, L., Kashirina, N., Sharf, T., Kuznetzova, I., Efremov, E., Yanushevskaya, E., Rutkevich, P., Rybalkin, I., & Vlasik, T. (2023). β1-Adrenergic Receptor Solubilized in the Form of Nanodiscs: Screening of Various Amphipatic PolymersBiomed Chem: Res Meth6(4), e00206. PDF

Zhang H, Shou C, Li CX, Pan J, & Xu, JH. Synthesis and modification of alternative copolymers for activating membrane enzymes in vitroEur Polym J200, 112485.

Nie YY, Yang GT, Wang HY, Qiao XQ. Modified styrene-maleic anhydride copolymer-based chromatographic stationary phase for phospholipid separation and analysis. Se Pu. 41(10):921-928. PDF

Stowe, R. A Spectroscopic and Biochemical Study of Protein Interactions and Membrane Mimetic Systems. PhD Thesis, Miami University. PDF

Cruz SJ, Burendei B, Lee WH, Ward A. Structure of mechanically activated ion channel OSCA2.3 reveals mobile elements in the transmembrane domain. bioRxiv. PDF

Knejski PP, Erramilli Sk, Kossiakoff AA.Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme uptake. bioRxiv. PDF

Morizumi T, Kim K, Li H, Govorunova EG, Sineshchekov OA, Wang Y, Zheng L, Bertalan É, Bondar AN, Askari A, Brown LS, Spudich JL, Ernst OP. Structures of channel rhodopsin paralogs in peptidiscs explain their contrasting K+ and Na+ selectivities. Nat Commun. 14(1):4365. PDF

Monjal V, Moreno A, Glueck D, S Keller S, S Guillet P, Durand G. Synthesis of well-defined methacrylate copolymers and their use for stabilizing membrane proteins. J Poly Sci, in press. PDF

Ye Y, Morita S, Chang JJ, Buckley PM, Wilhelm KB, DiMaio D, Groves JT, Barrera FN. Allosteric inhibition of the T cell receptor by a designed membrane ligand. Elife. 12, e82861 PDF

Sluchanko NN, Kapitonova AA, Shulepko MA, Kukushkin ID, Kulbatskii DS, Tugaeva KV, Varfolomeeva LA, Minyaev ME, Boyko KM, Popov VO, Kirpichnikov MP, Lyukmanova EN. Crystal structure reveals canonical recognition of the phosphorylated cytoplasmic loop of human alpha7 nicotinic acetylcholine receptor by 14-3-3 protein. Biochem Biophys Res Commun 682, 91-96. PDF

Krishnarjuna B, Sharma G, Im SC, Auchus R, Anantharamaiah GM, Ramamoorthy A. Characterization of nanodisc-forming peptides for membrane protein studies. J Colloid Interface Sci. 653, 1402-1414. PDF

Balachandra K. Structural Discovery of CF1F0 using Nanodiscs: exploring the ability of DIBMA to isolate spinach chloroplast ATP synthase. PhD Thesis, Arizona State University.

Wang T. Investigating the use of SMA-lipoparticles in the extraction and mass spectrometry-based analysis of membrane proteome and targeted membrane glycoprotein. Thesis, NTU

Chiu YC, Yeh MC, Wang CH, Chen YA, Chang H, Lin HY, Ho MC, Lin SM. Structural basis for calcium-stimulating pore formation of Vibrio α-hemolysin. Nat Commun. 14(1):5946. PDF

Woods H, Leman JK, Meiler J, Modeling membrane geometries implicitly in Rosetta - bioRxiv. PDF

Walters SH, Castillo AJ, Develin AM, Labrecque CL, Qu Y, Fuglestad B. Investigating protein-membrane interactions using native reverse micelles constructed from naturally sourced lipids. Protein Sci. e4786. PDF

Sarcinella M, Analytical Characterization and Biological Impacts of Multi-Lipid Nanodiscs. PhD Thesis, U Michigan. PDF

Zhang G, Odenkirk MT, Janczak CM, Lee R, Richardson K, Wang Z, Aspinwall CA, Marty MT. Identifying Membrane Protein-Lipid Interactions with Lipidomic Lipid Exchange-Mass Spectrometry. J Am Chem Soc. in press. PDF

Lai LTF, Balaraman J, Zhou F, Matthies D. Cryo-EM structures of human magnesium channel MRS2 reveal gating and regulatory mechanisms. bioRxiv. PDF

Margheritis E, Kappelhoff S, Cosentino K. Pore-Forming Proteins: From Pore Assembly to Structure by Quantitative Single-Molecule Imaging. Int J Mol Sci. 24(5):4528. PDF

Janata M, Gupta S, Čadová E, Angelisov P, Krishnarjuna B, Ramamoorthy A, Hořejší V, Raus V. Sulfonated polystyrenes: pH and Mg2+-insensitive amphiphilic copolymers for detergent-free membrane protein isolation. Eur Polymer J. 112412. PDF

Zhu Y, Odenkirk MT, Qiao P, Zhang T, Schrecke S, Zhou M, Marty MT, Baker ES, Laganowsky A. Combining native mass spectrometry and lipidomics to uncover specific membrane protein-lipid interactions from natural lipid sources. Chem Sci. 14(32):8570-8582. PDF

Matthews, HK. DNA Support Structures For Membrane Protein Imaging. PhD Thesis, Kent State University. PDF

Kefauver JM , Hakala M, Zou L, Alba J, Espadas Moreno J, Tettamanti MG, Estrozi LF, Vanni S, Roux A, Desfosses A, Loewith R. CryoEM architecture of a native stretch-sensitive membrane microdomain. bioRxiv. PDF

Campuzano IDG. A Research Journey: Over a Decade of Denaturing and Native-MS Analyses of Hydrophobic and Membrane Proteins in Amgen Therapeutic Discovery. J Am Soc Mass Spectrom. in press. PDF

Ryan BJ, Kinsella GK, Henehan GT. Protein Extraction and Purification by Differential Solubilization. Methods Mol Biol. 2699:349-368. PDF

Brust CA, Swanson MA, Bohn LM. Structural and functional insights into the G protein-coupled receptors: CB1 and CB2. Biochem Soc Trans. 51(4):1533-1543. PDF

Chen GL, Li J, Zhang J, Zeng B. To Be or Not to Be an Ion Channel: Cryo-EM Structures Have a Say. Cells. 12(14):1870. PDF

Yangyang N, Guantao Y, Haiyan W, Xiaoqiang Q, Modified styrene-maleic anhydride copolymer-based chromatographic stationary phase for phospholipid separation and analysis. Chin J Chromatogr. 年(月). PDF

Vénien-Bryan C, Fernandes CAH. Overview of Membrane Protein Sample Preparation for Single-Particle Cryo-Electron Microscopy Analysis. Preprints. PDF

Kim SJ, Koh YH, Roh SH. Unraveling membrane protein localization and stabilization in nanodiscs. bioRxiv. PDF

Köck Z, Schnelle K, Persechin M, Umbach S, Schihada H, Januliene D, Parey K, Pockes S, Kolb P, Dötsch V, Möller A, Hilger D, Bernhard F. Cryo-EM structure of cell-free synthesized human histamine H2 receptor coupled to 2 heterotrimeric Gs protein in lipid nanodisc environment. bioRxiv. PDF

Shree S, McLean MA, Stephen AG, Sligar SG. Revealing KRas4b topology on the membrane surface. Biochem Biophys Res Commun. 678:122-127. PDF

Workman CE.,The Development of Tailored Amphiphilic Copolymers for Detergent-Free Integral Membrane Protein Extraction , PhD Thesis. University of Tennessee, Knoxville.

Juliano BR, Keating JW, Ruotolo BT. Infrared Photoactivation Enables Improved Native Top-Down Mass Spectrometry of Transmembrane Proteins. Anal Chem. in press. PDF

Necelis M, McDermott C, Belcher Dufrisne M, Baryiames C, Columbus L. Solution NMR investigations of integral membrane proteins: Challenges and innovations. Curr Opin Struct Biol. 82:102654. PDF

Situ AJ, Ulmer TS. Comparison of Integrin αIIbβ3 Transmembrane Association in Vesicles and Bicelles. Biochemistry. 62(12):1858-1863. PDF

Sahoo BR, Ramamoorthy A. Direct interaction between the transmembrane helices stabilize cytochrome P450 2B4 and cytochrome b5 redox complex. Biophys Chem. 301, 107092. PDF

Dutta D. Interplay between membrane proteins and membrane protein-lipid pertaining to plant salinity stress. Cell Biochem Funct. 41(4):399-412. PDF

Townsend J. Analysis of Viroporins and Membrane Systems With Native Mass Spectrometry. PhD thesis, The University of Arizona. PDF

Hurst CH, Turnbull D, Xhelilaj K, Myles S, Pflughaupt RL, Kopischke M, Davies P, Jones S, Robatzek S, Zipfel C, Gronnier J, Hemsley PA. S-acylation stabilizes ligand-induced receptor kinase complex formation during plant pattern-triggered immune signaling. Curr Biol. 33(8):1588-1596. PDF

Dirnberger B, Korona D, Popovic R, Deery MJ , Barber H, Russell S, Lilley KS. Enrichment of Membrane Proteins for Downstream Analysis Using Styrene Maleic Acid Lipid Particles (SMALPs) Extraction, bioprotocol, in13(15):e4728. PDF

Hilton KLF, Tolley H, Ortega-Roldan JL, Thompson GS, Sutton JM, Hind CK,Hiscock JR, Phospholipid headgroup composition modulates the molecular interactions and antimicrobial effects of sulfobetaine zwitterionic detergents against the “ESKAPE” pathogen Pseudomonas aeruginosa.Chemical Communs. PDF

Müller WA, Beales PA, Muniz AR, Jeuken LJC. Unraveling the Phase Behavior, Mechanical Stability, and Protein Reconstitution Properties of Polymer-Lipid Hybrid Vesicles. Biomacromolecules. in press. PDF

Maslov I, Volkov O, Khorn P, Orekhov P, Gusach A, Kuzmichev P, Gerasimov A, Luginina A, Coucke Q, Bogorodskiy A, Gordeliy V, Wanninger S, Barth A, Mishin A, Hofkens J, Cherezov V, Gensch T, Hendrix J, Borshchevskiy V. Sub-millisecond conformational dynamics of the A2A adenosine receptor revealed by single-molecule FRET. Commun Biol. 6(1):362. PDF

Dodge GJ, Anderson AJ, He Y, Liu W, Viner R, Imperiali B, Mapping the architecture of the initiating phosphoglycosyl transferase from S. enterica O-antigen biosynthesis in a liponanoparticle, bioRxiv. PDF

Pettersen JM, Yang Y, Robinson AS. Advances in nanodisc platforms for membrane protein purification. Trends Biotechnol. 41(8):1041-1054. PDF

Scheyer MW, Campbell C, William PL, Hussain M, Begum A, Fonseca SE, Asare IK, Dabney P, Dabney-Smith C, Lorigan GA, Sahu ID. Electron paramagnetic resonance spectroscopic characterization of the human KCNE3 protein in lipodisq nanoparticles for structural dynamics of membrane proteins. Biophys Chem. 301:107080. PDF

Rottet S, Iqbal S, Xifaras R, Singer MT, Scott C, Deplazes E, Callaghan R. Biochemical interactions between the Atm1-like transporter from Novosphingobium aromaticivorans and heavy metals. Arch Biochem Biophys. 744:109696. PDF

Syrjänen JL, Epstein M, Gómez R, Furukawa H. Structure of human CALHM1 reveals key locations for channel regulation and blockade by ruthenium red. Nat Commun. 14(1):3821. PDF

Farrelly MD, Zhai J, Tiong AYJ, van 't Hag L, Yu HH, Li J, Martin LL, Thang SH. Membrane interaction and selectivity of novel alternating cationic lipid-nanodisc assembling polymers. Biomater Sci. in press. PDF

Mueller S, Kubicek J, Merino F, Hanisch P, Maertens B, Lackmann JW. The bigger picture: global analysis of solubilization performance of classical detergents versus new synthetic polymers utilizing shotgun proteomics. bioRxiv. PDF

David L, Borges JP, L. Hollingsworth R, Volchuk A, Jansen I, Steinberg BE, Wu. NINJ1 mediates plasma membrane rupture through formation of nanodisc-like rings. bioRxiv. PDF

Hoven L. Hydrophobic polyelectrolytes: An examination of their cooperative partitioning in a 2-phase oil/water system and lipid membrane interactions. PhD Thesis. Utrecht University. PDF

Gamage N, Cheruvara H, Harrison PJ, Birch J, Hitchman CJ, Olejnik M, Owens RJ, Quigley A. High-Throughput Production and Optimization of Membrane Proteins After Expression in Mammalian Cells. Methods Mol Biol. 2652:79-118. PDF

Trinh TKH, Cabezas AJ, Joshi S, Catalano C, Siddique AB, Qiu W, Deshmukh S, des Georges A, Guo Y. pH-tunable membrane-active polymers, NCMNP2a-x, and their potential membrane protein applications. Chem Sci. 14(26):7310-7326. PDF

Ge S, Wang C, Si M, Zhu Q, Shan Y, Li N, Wang Y, Wang H, Luo G, He H, He L. Site-Specific Covalent Immobilization of SMA-Stabilized ACE2 for SARS-CoV-2 Recognition and Drug Screening. ACS Appl Mater Interfaces. in press. PDF

Young JW. Recent advances in membrane mimetics for membrane protein research. Biochem Soc Trans. 51(3):1405-1416. PDF

Cheng D, Zhao L, Guan G, Lai H, Luo J. Synthesis of SMA by RAFT polymerization and its dispersion of TiO2 in aqueous solution. RSC Adv. 2023 Mar 7;13(11):7366-7371. PDF

Hammerschmid D, Calvaresi V, Bailey C, Russell Lewis B, Politis A, Morris M, Denbigh L, Anderson M, Reading E. Chromatographic Phospholipid Trapping for Automated H/D Exchange Mass Spectrometry of Membrane Protein-Lipid Assemblies. Anal Chem. 95(5):3002-3011. PDF

Young JW, Pfitzner E, Kukura P, Robinson CV. Characterisation of membrane protein interactions by peptidisc-mediated mass photometry. bioRxiv

Michon B, López-Sánchez U, Degrouard J, Nury H, Leforestier A, Rio E, Salonen A, Zoonens M. Role of surfactants in electron cryo-microscopy film preparation. Biophys J. 122(10):1846-1857. PDF

Zhao Z, Khurana A, Antony F, Young JW, Hewton KG, Brough Z, Zhong T, Parker SJ, Duong van Hoa F. A Peptidisc-based Survey of the Plasma Membrane Proteome of a Mammalian Cell. Mol Cell Proteomics.100588. PDF

Jia XM, Long YR, Yu XL, Chen RQ, Gong LK, Geng Y. Construction of stable membranal CMTM6-PD-L1 full-length complex to evaluate the PD-1/PD-L1-CMTM6 interaction and develop anti-tumor anti-CMTM6 nanobody. Acta Pharmacol Sin. ;44(5):1095-1104. PDF

Pang C, Chai J, Zhu P, Shanklin J, Liu Q, Structural mechanism of intracellular autoregulation of zinc uptake in ZIP transporters. Nature Comms 14: 3404. PDF

Zhou R, Zhang S, Nguyen HT, Ding H, Gaffney A, Kappes JC, Smith AB, Sodroski JG. Conformations of Human Immunodeficiency Virus Envelope Glycoproteins in Detergents and Styrene-Maleic Acid Lipid Particles. J Virol 97(6): e0032723. PDF

Chai J, Guo G, McSweeney SM, Shanklin J, Liu Q. Structural basis for enzymatic terminal C-H bond functionalization of alkanes. Nat Struct Mol Biol. 30(4):521-526. PDF

Venkataraghavan A, Kim H, Schwerdt JG,Gulyuk, AV, Singh A, Yingling YG, Tyerman SD, Hrmova M. Barley multifunctional aquaporin HvNIP2;1 permeates metalloids and mono-and oligo-saccharides. bioRxiv. PDF

Scherhag A, Räschle M, Unbehend N, Venn B, Glueck D, Mühlhaus T, Keller S, Pérez Patallo E, Zehner S, Frankenberg-Dinkel N. Characterization of a soluble library of the Pseudomonas aeruginosa PAO1 membrane proteome with emphasis on c-di-GMP turnover enzymes. microLife uqad028. PDF

Levental I, Lyman E. Regulation of membrane protein structure and function by their lipid nano-environment. Nat Rev Mol Cell Biol. 24(2):107-122. PDF

Biou, V. Lipid-membrane protein interaction visualised by cryo-EM: A review. BBA - Biomembranes 1865, 184068. PDF

Angerer N, Piller P, Semeraro EF, Keller S, Pabst G. Interaction of detergent with complex mimics of bacterial membranes. Biophys Chem. 296:107002. PDF

Sahu ID, Lorigan GA. Perspective on the Effect of Membrane Mimetics on Dynamic Properties of Integral Membrane Proteins. J Phys Chem B. 127(17):3757-3765. PDF

Bjørnestad VA, Soto-Bustamante F, Tria G, Laurati M, Lund R. Beyond the standard model of solubilization: Non-ionic surfactants induce collapse of lipid vesicles into rippled bilamellar nanodiscs. J Colloid Interface Sci. 2023 Jul;641:553-567. PDF

Buachi C, Thammachai C, Tighe BJ, Topham PD, Molloy R, Punyamoonwongsa P. Encapsulation of propolis extracts in aqueous formulations by using nanovesicles of lipid and poly(styrene-alt-maleic acid). Artif Cells Nanomed Biotechnol. 51(1):192-204. PDF

Soulié M, Deletraz A, Wehbie M, Mahler F, Bouchemal I, Le Roy A, Petit-Härtlein I, Keller S, Meister A, Pebay-Peyroula E, Breyton C, Ebel C, Durand G. Zwitterionic fluorinated detergents: From design to membrane protein applications. Biochimie. 205:40-52. PDF

Olivas TJ, Wu Y, Yu S, Luan L, Choi P, Guinn ED, Nag S, De Camilli PV, Gupta K, Melia TJ. ATG9 vesicles comprise the seed membrane of mammalian autophagosomes. J Cell Biol. 222(7):e202208088. PDF

Wang K, Zhang S, Go EP, Ding H, Wang WL, Nguyen HT, Kappes JC, Desaire H, Sodroski J, Mao Y. Asymmetric conformations of cleaved HIV-1 envelope glycoprotein trimers in styrene-maleic acid lipid nanoparticles. Commun Biol. 6(1):535. PDF

Odenkirk MT, Zhang G, Marrt MT. Do Nanodisc Assembly Conditions Affect Natural Lipid Uptake? J AM Soc Mass Spectrom. 34(9) 2006-2015. PDF

Guo Y, Karimullina E, Emde T, Otwinowski Z, Borek D, Savchenko A. Monomer and dimer structures of cytochrome bo3 ubiquinol oxidase from Escherichia coli. Protein Sci. 32(4):e4616. PDF

Reid DJ, Thibert S, Zhou M. Dissecting the structural heterogeneity of proteins by native mass spectrometry. Protein Sci. 32(4):e4612. PDF

Javed W, Griffiths D, Politis A. Hydrogen/deuterium exchange-mass spectrometry of integral membrane proteins in native-like environments: current scenario and the way forward. Essays Biochem. 67(2):187-200. PDF

Russell Lewis B, Lawrence R, Hammerschmid D, Reading E. Structural mass spectrometry approaches to understand multidrug efflux systems. Essays Biochem. 67(2):255-267. PDF

Drewniak, P. Structural studies of human Aquaporin-1 in polymer nanodiscs and an investigation into a conserved hydrogen-bond network crucial for stability. MSc Thesis, University of Guelph. PDF

Sanders HM, Chalyavi F, Fields CR, Kostelic MM, Li MH, Raleigh DP, Zanni MT, Marty MT. Interspecies Variation Affects Islet Amyloid Polypeptide Membrane Binding. J Am Soc Mass Spectrom. 7;34(6):986-990. PDF

Zhou Y, Syed JH, Semchonok DA, Wright E, Kyrilis FL, Hamdi F, Kastritis PL, Bruce BD, Reynolds TB. Solubilization, purification, and characterization of the hexameric form of phosphatidylserine synthase from Candida albicans. J Biol Chem. 299,6:104756. PDF

Nadezhdin KD, Correia L, Narangoda C, Patel DS, Neuberger A, Gudermann T, Kurnikova MG, Chubanov V & Sobolevsky AI, Structural mechanisms of TRPM7 activation and inhibition. Nature Communs 14: 2639. PDF

Kojima K, Kawanishi S, Nishimura Y, Hasegawa M, Nakao S, Nagata Y, Yoshizawa S, Sudo Y. A blue-shifted anion channelrhodopsin from the Colpodellida alga Vitrella brassicaformis. Sci Rep. 13(1):6974. PDF

Dallo S, Shin J, Zhang S, Ren Q, Bao H. Designer Nanodiscs to Probe and Reprogram Membrane Biology in Synapses. J Mol Biol. 435(1):167757. PDF

Tao X, Zhao C, MacKinnon R. Membrane protein isolation and structure determination in cell-derived membrane vesicles. PNAS USA 120(18):e2302325120. PDF

Robinson JLM, Kegel WK. Cooperative transitions involving hydrophobic polyelectrolytes. PNAS USA 120(6):e2211088120. PDF

Harrison PJ, Vecerkova T, Clare DK, Quigley A. A review of the approaches used to solve sub-100 kDa membrane proteins by cryo-electron microscopy. J Struct Biol. 215 (2):107959. PDF

Clark JC, Martin EM, Morán LA, Di Y, Wang X, Zuidscherwoude M, Brown HC, Kavanagh DM, Hummert J, Eble JA, Nieswandt B, Stegner D, Pollitt AY, Herten DP, Tomlinson MG, García A, Watson SP. Divalent nanobodies to platelet CLEC-2 can serve as agonists or antagonists. Commun Biol. 6(1):376. PDF

Rejnowicz E, Wright J, Veldman-Jones M, Harborne S. Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography. J Vis Exp. 191. PDF

Lu J, Zhang C, Shi S, Li S, Liu J, Wu J, Huang C, Lei M. Stoichiometry and architecture of the platelet membrane complex glycoprotein Ib-IX-V. Biol Chem. in press.

Kedare MM, Hazra S, Satyam S, Patra S. Recent Developments in Protein Extraction Methods from Bacteria, Yeast and Natural Sources for Various Applications SSRN. PDF

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Jamshad M, Charlton J, Lin YP, Routledge SJ, Bawa Z, Knowles TJ, Overduin M, Dekker N, Dafforn TR, Bill RM, Poyner DR, Wheatley M. G-protein coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent. Biosci Rep 35 pii: e00188. PMID: 25720391. PDF


Jamshad M, Grimard V, Idini I, Knowles TJ, Dowle MR, Schofield N, Sridhar P, Lin YP, Finka R, Wheatley M, Thomas ORT, Palmer RE, Overduin M, Govaerts C, Ruysschaert JM, Edler KJ, Dafforn TR. Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins. Nano Research 8: 774-789. PDF

Paulin S, Jamshad M, Dafforn TR, Garcia-Lara J, Foster SJ, Galley NF, Roper DI, Rosado H, Taylor PW. Surfactant-free purification of membrane protein complexes from bacteria: application to the staphylococcal penicillin-binding protein complex PBP2/PBP2a. Nanotechnology 25:285101. PMID: 24972373. PDF

Swainsbury DJ, Scheidelaar S, van Grondelle R, Killian JA, Jones MR. Bacterial reaction centers purified with styrene maleic acid copolymer retain native membrane functional properties and display enhanced stability. Angew Chem Int Ed Engl 53(44):11803-7. PMID: 25212490. PDF

Dörr JM, Koorengevel MC, Schäfer M, Prokofyev AV, Scheidelaar S, van der Cruijsen EA, Dafforn TR, Baldus M, Killian JA. Detergent-free isolation, characterization, and functional reconstitution of a tetrameric K+ channel: the power of native nanodiscs. Proc Natl Acad Sci USA 111(52):18607-12. PMID: 25512535. PDF

Sahu ID, Kroncke BM, Zhang R, Dunagan MM, Smith HJ, Craig A, McCarrick RM, Sanders CR, Lorigan GA. Structural investigation of the transmembrane domain of KCNE1 in proteoliposomes. Biochemistry 53:6392-401. PMID: 25234231. PDF

Malhotra K, Alder NN. Advances in the use of nanoscale bilayers to study membrane protein structure and functionStructural investigation of the transmembrane domain of KCNE1 in proteoliposomes. Biotechnol Genet Eng Rev 30:79-93. PMID: 25023464. PDF


Gulati S, Jamshad M, Knowles TJ, Morrison KA, Downing R, Cant N, Collins R, Koenderink JB, Ford RC, Overduin M, Kerr ID, Dafforn TR, Rothnie AJ. Detergent-free purification of ABC (ATP-binding-cassette) transporters. Biochem J 461:269-78. PMID: 24758594. PDF

Sahu ID, McCarrick RM, Troxel KR, Zhang R, Smith HJ, Dunagan MM, Swartz MS, Rajan PV, Kroncke BM, Sanders CR, Lorigan GA. DEER EPR measurements for membrane protein structures via bifunctional spin labels and lipodisq nanoparticles. Biochemistry 52:6627-32. PMID: 23984855. PDF

Long AR, O'Brien CC, Malhotra K, Schwall CT, Albert AD, Watts A, Alder NN. A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs. BMC Biotechnol 13:41. PMID: 23663692. PDF


Orwick MC, Judge PJ, Procek J, Lindholm L, Graziadei A, Engel A, Gröbner G, Watts A. Detergent-free formation and physicochemical characterization of nanosized lipid-polymer complexes: Lipodisq. Angew Chem Int Ed Engl 51:4653-7. PMID: 22473824. PDF

Orwick-Rydmark M, Lovett JE, Graziadei A, Lindholm L, Hicks MR, Watts A. Detergent-free incorporation of a seven-transmembrane receptor protein into nanosized bilayer Lipodisq particles for functional and biophysical studies. Nano Lett 12:4687-92. PMID: 22827450. PDF

Banerjee S, Pal TK, Guha SK. Probing molecular interactions of poly(styrene-co-maleic acid) with lipid matrix models to interpret the therapeutic potential of the co-polymer. Biochim Biophys Acta. 1818(3):537-50. 22197504. PDF

Dafforn TR, Thomas ORT. Protein extraction, Patent GB20110007737 20110509; WO2012GB00423 20120509. PDF


Jamshad M, Lin YP, Knowles TJ, Parslow RA, Harris C, Wheatley M, Poyner DR, Bill RM, Thomas OR, Overduin M, Dafforn TR. Surfactant-free purification of membrane proteins with intact native membrane environment. Biochem Soc Trans 39:813-8. PMID: 21599653. PDF

Rajesh S, Knowles T, Overduin M. Production of membrane proteins without cells or detergents. N Biotechnol 28:250-4. PMID: 20654746. PDF


Overduin M, Jamshad M, Lin Y, Knowles T, Jazayeri A, Poyner D, Bill R, Wheatley M, Dafforn T. Amphipathic polymer-based nanoparticles for purifying and characterizing stable and active GPCRs without detergents. Keystone Symposium on G Protein-Coupled Receptors.

Overduin M, Knowles T, Dafforn T. Solubilisation of membrane proteins. Patent GB2010/001309; WO2011/004158. PDF


Knowles TJ, Finka R, Smith C, Lin YP, Dafforn T, Overduin M. Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer. J Am Chem Soc 131:7484-5. PMID: 19449872. PDF


Tonge S. Compositions comprising a lipid and copolymer of styrene and maleic acid (note that the membrane protein data included in this patent is from Knowles et. al., 2009). Patent PCT/GB2006/050134; WO2006/129127. PDF

Overduin, M, Tonge S. Memtein: Providing Membrane Protein Solutions, Venturefest finalist at the Oxford University Business Plan Competition.


Michael Overduin submits Nanodisks for Drug Screening to Birmingham Research and Development Ltd, and is awarded a Technology Transfer Fund Grant by the Central Technology Belt for commercial development of the SMALP system with Steve Tonge being a co-applicant.


A Royal Society Wolfson Research Merit Award to Michael Overduin entitled Structural Biology of Membrane Spanning Proteins by NMR Spectroscopy is funded from 2004-2009.